细胞角蛋白18抗体Anti-CK18齐一生物科技（上海）有限公司产品已被广泛应用于化学、化工、生命科学的基础研究和开发应用、制药、疾病诊断与控制、人口与健康、生物技术等诸多领域.客户遍布国内各大学、研究所、医院、卫生防疫、商品检验检疫、制药公司、生物技术公司和食品工业等单位.齐一生物科技（上海）有限公司：.www.qiyibio。。ｃｏｍ

细胞角蛋白18抗体Anti-CK18英文名称    CK18
中文名称    细胞角蛋白18抗体
别    名    Cell proliferation inducing gene 46 protein; Cell proliferation inducing protein 46; CK 18; CK18; CYK18; Cytokeratin 18; Cytokeratin endo B; Cytokeratin18; Keratin 18; Keratin D; Keratin type I cytoskeletal 18; Kerd; KRT18.
研究领域    肿瘤  免疫学  信号转导   
抗体来源    Rabbit
克隆类型    Polyclonal
交叉反应    Human, Mouse, Rat, Chicken, Dog, Rabbit, 
产品应用    WB=1:100-500 ELISA=1:500-1000 IP=1:20-100 IHC-P=1:100-500 IHC-F=1:100-500 IF=1:100-500  （石蜡切片需做抗原修复） 
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.
分 子 量    48kDa
性    状    Lyophilized or Liquid
浓    度    1mg/1ml
免 疫 原    KLH conjugated synthetic peptide derived from human CK18
亚    型    IgG
纯化方法    affinity purified by Protein A
储 存 液    0.01M PBS, pH 7.4 with 10 mg/ml BSA and 0.1% Sodium azide
保存条件    Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C.
PubMed    PubMed
产品介绍    background:
KRT18 encodes the type I intermediate filament chain keratin 18. Keratin 18, together with its filament partner keratin 8, are perhaps the most monly found members of the intermediate filament gene family. They are expressed in single layer epithelial tissues of the body. Mutations in this gene have been linked to cryptogenic cirrhosis. Two transcript variants encoding the same protein have been found for this gene. [provided by RefSeq].

Function:
When phosphorylated, plays a role in filament reorganization. Involved in the delivery of mutated CFTR to the plasma membrane. Involved in the uptake of thrombin-antithrombin plexes by hepatic cells (By similarity). Together with KRT8, is involved in interleukin-6 (IL-6)-mediated barrier protection.

Post-translational modifications:
Phosphorylation increases by IL-6.
Proteolytically cleaved by caspases during epithelial cell apoptosis. Cleavage occurs at Asp-231 by either caspase-3, caspas-6 or caspase-7. 
O-glycosylated at multiple sites; glycans consist of single N-acetylglucosamine residue.

Similarity:
Belongs to the intermediate filament family.

Gene ID:
3875

Database links:
Entrez Gene: 506480 Cow
Entrez Gene: 3875 Human
Entrez Gene: 16668 Mouse
Entrez Gene: 294853 Rat
Omim: 148070 Human
SwissProt: P05783 Human
SwissProt: P05784 Mouse
SwissProt: Q5BJY9 Rat
Unigene: 406013 Human
Unigene: 22479 Mouse
Unigene: 103924 RatImportant Note:

This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. 

结构蛋白（Structural Proteins）
常用于肿瘤细胞的分化、增殖及转移方面的研究。
   Tissue/cell: human lung carcinoma; 4% Paraformaldehyde-fixed and paraffin-embedded; 
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min; 
Incubation: Anti-CK18 Polyclonal Antibody, Unconjugated(bs-1339R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining