HY-13757A-Tamoxifen试剂-杭州昊鑫生物科技股份有限公司手机版

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注：本产品仅用于科研，不可用于临床

Tamoxifen试剂

Tamoxifen (ICI 47699) 是一种口服有效的，选择性雌激素受体 (estrogen receptor) 调节剂 (SERM)，可阻断乳腺细胞中的雌激素作用，并可激活其他细胞，如骨骼，肝脏和子宫细胞中的雌激素活性。Tamoxifen 是一种有效的 Hsp90 激活剂，可增强 Hsp90 分子伴侣 ATPase 的活性。Tamoxifen 还可以有效抑制传染性 EBOV Zaire 和 Marburg (MARV)，IC₅₀ 分别为 0.1 µM 和 1.8 µM。Tamoxifen 可以激活自噬 (autophagy)，诱导凋亡 (apoptosis)。Tamoxifen 还可以诱导 CreER(T2) 转基因小鼠的基因敲除。

Tamoxifen试剂 生物活性

Tamoxifen (ICI 47699) is an orally active, selective estrogen receptor modulator (SERM) which blocks estrogen action in breast cells and can activate estrogen activity in other cells, such as bone, liver, and uterine cells^[1]^[2]^[3]. Tamoxifen is a potent Hsp90 activator and enhances the Hsp90 molecular chaperone ATPase activity. Tamoxifen also potent inhibits infectious EBOV Zaire and Marburg (MARV) with IC₅₀ of 0.1 µM and 1.8 µM, respectively^[5]. Tamoxifen activates autophagy and induces apoptosis^[4]. Tamoxifen also can induce gene knockout of CreER(T2) transgenic mouse^[6].

体外研究
(In Vitro)

Tamoxifen (ICI 47699) shows strong inhibition of MCF-7 cells (EC₅₀=1.41 μM) and to a lesser extent the T47D cells (EC₅₀=2.5 μM) but does not affect the MDA-MB-231 cells^[2].

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

体内研究
(In Vivo)

Injection of pre-mutant mice with Tamoxifen (75 mg/kg; injected for 5 days at 6 weeks of age) results in the excision of the floxed exon and, thus, in a gene knockout^[3].

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model:	Aldh1l1-cre/ERT2 x Ai95 mice^[3]
Dosage:	75 mg/kg
Administration:	Injected for 5 days at 6 weeks of age
Result:	Resulted in the excision of the floxed exon and a gene knockout.

分子量

371.51

性状

Solid

Formula

C₂₆H₂₉NO

CAS 号

10540-29-1

中文名称

三苯氧胺；它莫西芬

运输条件

Room temperature in continental US; may vary elsewhere.

储存方式

4°C, protect from light

*In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)

溶解性数据

In Vitro:

DMSO : 50 mg/mL (134.59 mM; Need ultrasonic)

Ethanol : 50 mg/mL (134.59 mM; Need ultrasonic)

配制储备液

浓度溶剂体积质量	1 mg	5 mg	10 mg

1 mM	2.6917 mL	13.4586 mL	26.9172 mL
5 mM	0.5383 mL	2.6917 mL	5.3834 mL
10 mM	0.2692 mL	1.3459 mL	2.6917 mL

请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液；一旦配成溶液，请分装保存，避免反复冻融造成的产品失效。
储备液的保存方式和期限：-80°C, 6 months; -20°C, 1 month (protect from light)。-80°C 储存时，请在 6 个月内使用，-20°C 储存时，请在 1 个月内使用。

In Vivo:

请根据您的实验动物和给药方式选择适当的溶解方案。以下溶解方案都请先按照 In Vitro 方式配制澄清的储备液，再依次添加助溶剂：

——为保证实验结果的可靠性，澄清的储备液可以根据储存条件，适当保存；体内实验的工作液，建议您现用现配，当天使用；以下溶剂前显示的百
分比是指该溶剂在您配制终溶液中的体积占比；如在配制过程中出现沉淀、析出现象，可以通过加热和/或超声的方式助溶

1.

请依序添加每种溶剂： corn oil
Solubility: 40 mg/mL (107.67 mM); Clear solution; Need ultrasonic
2.

请依序添加每种溶剂： 30% PEG400 0.5% Tween80 5% Propanediol 64.5%H2O
Solubility: 5 mg/mL (13.46 mM); Suspended solution; Need ultrasonic
3.

请依序添加每种溶剂： 10% EtOH 40% PEG300 5% Tween-80 45% saline
Solubility: 2.5 mg/mL (6.73 mM); Suspended solution; Need ultrasonic
4.

请依序添加每种溶剂： 10% EtOH 90% corn oil
Solubility: ≥ 2.5 mg/mL (6.73 mM); Clear solution
5.

请依序添加每种溶剂： 10% DMSO 40% PEG300 5% Tween-80 45% saline
Solubility: ≥ 2.08 mg/mL (5.60 mM); Clear solution
6.

请依序添加每种溶剂： 10% DMSO 90% (20% SBE-β-CD in saline)
Solubility: 2.08 mg/mL (5.60 mM); Suspended solution; Need ultrasonic
7.

请依序添加每种溶剂： 10% DMSO 90% corn oil
Solubility: ≥ 2.08 mg/mL (5.60 mM); Clear solution
8.

请依序添加每种溶剂： 5% DMSO 40% PEG300 5% Tween-80 50% saline
Solubility: ≥ 0.83 mg/mL (2.23 mM); Clear solution
9.

请依序添加每种溶剂： 5% DMSO 95% (20% SBE-β-CD in saline)
Solubility: 0.83 mg/mL (2.23 mM); Suspended solution; Need ultrasonic

10 mM * 1 mL in DMSO	550元	10000 可售
500 mg	500元	10000 可售
1 g	690元	10000 可售
5 g	2750元	10000 可售

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