HY-D1056-Lipopolysaccharides试剂-杭州昊鑫生物科技股份有限公司手机版

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注：本产品仅用于科研，不可用于临床

Lipopolysaccharides试剂生物活性

ipopolysaccharides (LPS)是一种内毒素来源于外层膜的外膜革兰氏阴性细菌。脂多糖由抗原O-specific链,一个核心的低聚糖和脂质a脂多糖是一种病原相关分子模式(PAMP时),激活免疫系统。脂多糖对免疫细胞激活地[1][2][3]。这个产品来源于大肠杆菌O55: B5。脂多糖诱导的分泌细胞migrasome

Lipopolysaccharides试剂 IC₅₀ & Target

TLR4

体外研究
(In Vitro)

Lipopolysaccharides (10–80 μg/mL) selectively decreases THir (tyrosine hydroxylase immunoreactive) cells and increases culture media levels of interleukin1β (IL-1β) and tumor necrosis factor-α (TNF-α) as well as nitrite (an index of nitric oxide (NO) production)^[5].

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

体内研究
(In Vivo)

Lipopolysaccharides (1.5 mg/kg; i.p.; once) induces sickness and hypothermia in mice, and induces a greater and more prolonged sickness response in adult male mice^[3].

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model:	Female and male CD1 mice^[3]
Dosage:	1.5mg/kg
Administration:	Intraperitoneal injection, once
Result:	Induced sickness behavior in all mice, but adult mice displayed more sickness than pubertal mice and adult males remained sick for a longer period of time than adult females. Caused a decrease in body temperature for all mice, but this decrease was greatest in adult males. Increased pro- and anti-inflammatory cytokines at various levels in pubertal and adult male and female mice, resulted in age and sex differences in cytokine concentrations following immune challenge. Only adult males and females treated with LPS displayed significantly more IL-6 than their saline controls, and pubertal males and females and adult females displayed significantly more IL-10 than their saline controls. All the mice displayed significantly more IL-12 and TNF-α than their saline controls.

性状

Solid

中文名称

脂多糖

结构分类

Saccharides

初始来源

surface of Gram-negative bacteria

运输条件

Room temperature in continental US; may vary elsewhere.

储存方式

Powder	-20°C	3 years
	4°C	2 years
In solvent	-80°C	6 months
	-20°C	1 month

溶解性数据

In Vitro:

H₂O : 5 mg/mL (Need ultrasonic)

In Vivo:

请根据您的实验动物和给药方式选择适当的溶解方案。以下溶解方案都请先按照 In Vitro 方式配制澄清的储备液，再依次添加助溶剂：

——为保证实验结果的可靠性，澄清的储备液可以根据储存条件，适当保存；体内实验的工作液，建议您现用现配，当天使用；以下溶剂前显示的百
分比是指该溶剂在您配制终溶液中的体积占比；如在配制过程中出现沉淀、析出现象，可以通过加热和/或超声的方式助溶

1.

请依序添加每种溶剂： PBS
Solubility: 8.33 mg/mL (Infinity mM); Clear solution; Need ultrasonic and warming and heat to 60°C

5 mg	1320元	10000 可售
10 mg	2200元	10000 可售
25 mg	4800元	10000 可售

产品简介

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