HY-13948-Angiotensin II human-杭州昊鑫生物科技股份有限公司手机版

详细介绍

注：本产品仅用于科研，不可用于临床

生物活性

血管紧张素ⅱ(血管紧张素ⅱ)是一种血管收缩剂和肾素-血管紧张素系统的主要生物活性肽。血管紧张素ⅱ人类中起着重要的作用在调节人体血压,主要由血管紧张素ⅱ和G-protein-coupled受体之间的相互作用(GPCRs)血管紧张素ⅱ1型受体(AT1R)和血管紧张素ⅱ2型受体(AT2R)。血管紧张素ⅱ人类刺激交感神经的刺激,增加醛固酮生物合成和肾的行动。人类血管紧张素ⅱ诱导的血管平滑肌细胞,增加胶原蛋白I型和III在成纤维细胞合成,导致血管壁增厚和心肌纤维化。血管紧张素ⅱ人类也诱发细胞凋亡。血管紧张素ⅱ诱导形成毛细血管内皮细胞通过LOX-1依赖redox-sensitive途径。.

IC₅₀ & Target



AT1 Receptor	AT2 Receptor

体外研究
(In Vitro)

大多数已知的血管紧张素ⅱ(Ang II)行动由AT1受体介导,at₂受体有助于调节血压和肾脏功能[1]
. Angiotensin II raises blood pressure (BP) by a number of actions, the most important ones being vasoconstriction, sympathetic nervous stimulation, increased aldosterone biosynthesis and renal actions. Other Angiotensin II actions include induction of growth, cell migration, and mitosis of vascular smooth muscle cells, increased synthesis of collagen type I and III in fibroblasts, leading to thickening of the vascular wall and myocardium, and fibrosis. These actions are mediated by type 1 Ang II receptors (AT₁)^[2].
Angiotensin II (1 nM) induces the expression of LOX-1 and VEGF and enhances capillary formation from human coronary endothelial cells in Matrigel assay. Angiotensin II -mediated expression of LOX-1 and VEGF, capillary formation, intracellular reactive oxygen species generation, and phosphorylation of p38 as well as p44/42 mitogen-activated protein kinases, are suppressed by anti-LOX-1 antibody, nicotinamide-adenine dinucleotide phosphate oxidase inhibitor apocynin and the Ang II type 1 receptor blocker Losartan, but not by the Ang II type 2 receptor blocker PD123319^[3].

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

体内研究
(In Vivo)

Angiotensin II human (5 mL of 1 nM; intraperitoneal injection; 200-250 g Sprague-Dawley rats) induces a significant neutrophil recruitment that was maximal at 4 hours and had resolved by 24 hours^[4].
To distinguish the AT₁ receptor population that is critical for the pathogenesis of hypertension, osmotic minipumps are implanted s.c. into each animal to infuse Angiotensin II (1000 ng/kg/min) continuously for 4 weeks. Angiotensin II causes hypertension by activating AT₁ receptors in the kidney promoting sodium reabsorption^[5].

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

分子量

1046.18

性状

Solid

Formula

C₅₀H₇₁N₁₃O₁₂

CAS 号

4474-91-3

Sequence

Asp-Arg-Val-Tyr-Ile-His-Pro-Phe

Sequence Shortening

DRVYIHPF

中文名称

血管紧张素Ⅱ

结构分类

Others

运输条件

Room temperature in continental US; may vary elsewhere.

储存方式

Sealed storage, away from moisture and light, under nitrogen

Powder	-80°C	2 years
	-20°C	1 year

*In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light, under nitrogen)

溶解性数据

In Vitro:

DMSO : 100 mg/mL (95.59 mM; Need ultrasonic)

H₂O : 50 mg/mL (47.79 mM; Need ultrasonic)

配制储备液

浓度溶剂体积质量	1 mg	5 mg	10 mg

1 mM	0.9559 mL	4.7793 mL	9.5586 mL
5 mM	0.1912 mL	0.9559 mL	1.9117 mL
10 mM	0.0956 mL	0.4779 mL	0.9559 mL

请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液；一旦配成溶液，请分装保存，避免反复冻融造成的产品失效。
储备液的保存方式和期限：-80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light, under nitrogen)。-80°C 储存时，请在 6 个月内使用，-20°C 储存时，请在 1 个月内使用。

In Vivo:

请根据您的实验动物和给药方式选择适当的溶解方案。以下溶解方案都请先按照 In Vitro 方式配制澄清的储备液，再依次添加助溶剂：

——为保证实验结果的可靠性，澄清的储备液可以根据储存条件，适当保存；体内实验的工作液，建议您现用现配，当天使用；以下溶剂前显示的百
分比是指该溶剂在您配制终溶液中的体积占比；如在配制过程中出现沉淀、析出现象，可以通过加热和/或超声的方式助溶

1.

请依序添加每种溶剂： PBS
Solubility: 30 mg/mL (28.68 mM); Clear solution; Need ultrasonic
2.

请依序添加每种溶剂： 10% DMSO 40% PEG300 5% Tween-80 45% saline
Solubility: ≥ 2.5 mg/mL (2.39 mM); Clear solution
3.

请依序添加每种溶剂： 10% DMSO 90% corn oil
Solubility: ≥ 2.5 mg/mL (2.39 mM); Clear solution
4.

请依序添加每种溶剂： 10% DMSO 90% (20% SBE-β-CD in saline)
Solubility: ≥ 0.83 mg/mL (0.79 mM); Clear solution

10 mg	825元	10000 支可售
50 mg	3069元	10000 支可售
100 mg	5500元	10000 支可售

产品简介

详细介绍