6-TAMRA Azide|6-TAMRA N3

6-TAMRA Azide (also known as TAMRA azide, isomer 6) is the red-fluorescent probe that is patible with various excitation sources including mercury arc, tungsten and xenon arc lamps, the 544 nm line of the Helium-Neon laser and the 532 nm green laser line. It is predominantly used for detection of terminal alkyne-tagged biomolecules via a copper-catalyzed click reaction (CUAAC). It also reacts with strained cyclooctyne via a copper-free &lduo;click chemistry&rduo; reaction to form a stable triazole and does not reuire Cu-catalyst or elevated temperatures.

Although the mixed isomers of 5(6)-TAMRA azide is a preferred, routinely used red fluorescent dye for staining proteins, labeling peptides and nucleotides with TAMRA mixed isomers might be troublesome due to significant signal broadening in HPLC purification. Peptides and nucleotides labeled with a single isomer TAMRA usually give better resolution in HPLC purification that is often reuired in the conjugation processes.

6-TAMRA Azide|6-TAMRA N3

• 叠氮化合物(Azides)
  Azidobutyric acid NHS ester
• Azido dyes, available as solids, and as ready-to-use 10 mM DMSO solutions
• Water-soluble Cy3 azide
  Water-soluble Cy5 azide
  Cy3 azide
  Cy3.5 azide
  Cy5 azide
  Cy5.5 azide
  Cy7 azide
  Cy7.5 azide
  5-FAM azide
  6-FAM azide
  5-JOE azide
  5-R110 azide
  6-R110 azide
  6-ROX azide
  5-TAMRA azide
  炔烃类染料(Alkynes)
  Alkyne phosphoramidite, 5'-terminal
  Alkyne amidite, hydroxyprolinol
  Pentynoic acid STP ester
  荧光激活染料(Catalysts and activators)
  Ascorbic acid
  Copper(II)-TBTA plex, 10 mM in 55% a. DMSO
  
  SYBR Green I
  Gel staining solution, 10000x
  For PCR, 100x solution
  
  改良三磷酸盐(Modified triphosphates)
  Ami1-ddUTP
  Ami1-dUTP
  
  脂类活性染料(Dye activated esters)
  Cy3 NHS ester (water soluble)
  Cy5 NHS ester (water soluble)
  Cy3 NHS ester
  Cy3.5 NHS ester
  Cy5 NHS ester
  Cy5.5 NHS ester
  Cy7 NHS ester
  Cy7.5 NHS ester
  ROX NHS ester, pure 6- isomer
  
  羰基活性染料(Dye hydrazides)
  Cy5 hydrazide
  
  巯基反应性染料(Dye maleimides)
  Cy5 maleimide
  Cy5.5 maleimide
  
  2D DIGE荧光染料试剂盒(2D DIGE dye kits)
  Phosporamidites
  Alkyne phosphoramidite, 5'-terminal
  Alkyne amidite, hydroxyprolinol
  DMS(O)MT aminolink amidite
  Long trebler amidite
  Pyrene dU amidite