5-TAMRA Azide|5-TAMRA N3|1006592-61-5

1006592-61-55-TAMRA Azide|5-TAMRA N3|1006592-61-5

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5-TAMRA Azide|5-TAMRA N3|1006592-61-5
TAMRA 荧光染料是在用的多种不同共轭物中Z受欢迎的荧光试剂之一,可标记蛋白质和寡核苷酸。TAMRA具有吸光的性质,它的光谱与几类常用的荧光化合物重叠,TMR是明亮的橙色荧光基团。

详细介绍

5-TAMRA Azide|5-TAMRA N3|1006592-61-5

 

5-TAMRA Azide (also known as TAMRA azide, isomer 5) is the red-fluorescent probe that is patible with various excitation sources including mercury arc, tungsten and xenon arc lamps, the 544 nm line of the Helium-Neon laser and the 532 nm green laser line. It is predominantly used for detection of terminal alkyne-tagged biomolecules via a copper-catalyzed click reaction (CUAAC). It also reacts with strained cyclooctyne via a copper-free &lduo;click chemistry&rduo; reaction to form a stable triazole and does not reuire Cu-catalyst or elevated temperatures.

Although the mixed isomers of 5(6)-TAMRA azide is a preferred, routinely used red fluorescent dye for staining proteins, labeling peptides and nucleotides with TAMRA mixed isomers might be troublesome due to significant signal broadening in HPLC purification. Peptides and nucleotides labeled with a single isomer TAMRA usually give better resolution in HPLC purification that is often reuired in the conjugation processes.

5-TAMRA azide is exact replacement of Invitrogen&rsuo;s (Thermo Fisher Scientific) Tetramethylrhodamine (TAMRA) Azide (Tetramethylrhodamine 5-Carboxamido-(6-Azidohexanyl)), 5-isomer

 

               

Abs/Em Maxima

553/575

Extinction coefficient

92,000

Flow cytometry laser line

532 or 555 nm

Microscopy laser line

532 or 555 nm

Spectrally similar dyes

Alexa Fluor® 546, Atto™ 543, CF™ 555 Dye, DyLight™549

Molecular weight

555.67

CAS

1006592-61-5

Solubility

Water, DMSO, DMF, MeOH

Purity

>98% (HPLC)

Appearance

Red solid

Storage conditions

-20C

Shipping conditions

Ambient temperature

5-TAMRA Azide|5-TAMRA N3|1006592-61-5

 

其他罗丹明荧光染料试剂:

5(6)-Carboxy tetramethyl rhodamine;5(6)-TAMRA                     150347-56-1

5-Carboxytetramethylrhodamine; 5-TAMRA                                     91809-66-4

6-Carboxytetramethylrhodamine;6-TAMRA              91809-67-5

&uot;5(6)-Carboxytetramethylrhodamine succinimidyl ester;5(6)-TAMRA, SE&uot;  246256-50-8

&uot;5-Carboxytetramethylrhodamine succinimidyl ester;5-TAMRA, SE&uot;              150810-68-7

&uot;6-Carboxytetramethylrhodamine succinimidyl ester;6-TAMRA, SE&uot;              150810-69-8

&uot;1,4-dichloro 5-Carboxytetramethylrhodamine&uot;                               198546-47-3

&uot;1,4-dichloro 6-Carboxytetramethylrhodamine&uot;                               407581-83-3

Sulforhodamine 101; Sulforhodamine 640                                   60311-02-6

5-Carboxy-X- rhodamine; 5-ROX                                         216699-35-3

6-Carboxy-X- rhodamine; 6-ROX                                         194785-18-7

5(6)-Carboxy-X- rhodamine; 5(6)-ROX                                          198978-94-8

6 d ROX                            

5 d ROX                            

Rhodamine 101;Rhodamine 640                                            64339-18-0

 

5(6)-TAMRA C6 maleimide 

5-TAMRA C6 maleimide      

6-TAMRA C6 maleimide                    

5(6)-TAMRA Maleimide                           

5-TAMRA Maleimide                        

6-TAMRA Maleimide                               

5-TAMRA alkyne                              

6-TAMRA alkyne                       

5-TAMRA azide                                

6-TAMRA azide                                

5(6)-TAMRA cadaverine                                  

5-TAMRA cadaverine                               

6-TAMRA cadaverine                               

5(6)-TAMRA ethylenediamine                          

5-TAMRA ethylenediamine                       

6-TAMRA ethylenediamine                              

 

 

 

 

 

5-TAMRA TEA Salt     (5-羧基四甲基罗丹明三乙胺盐)          150322-05-7                

5-TAMRA-NHS        (5-羧基四甲基罗丹明琥珀酰亚胺酯)      3212-17-3                

6-TAMRA TEA salt     (6-羧基四甲基罗丹明三乙胺盐)           150322-06-8                

6-Aminotetramethylrhodamine     (6-氨基四甲基罗丹明)            80724-18-1                  

5-Aminotetramethylrhodamine    (5-氨基四甲基罗丹明)              80724-17-0,167095-10-5     

6-FAM-C6-OH    6-(N-6-羟基己基)酰胺荧光素                467456-19-5                

5(6)-FAM-C6-OH   5/6-(N-6-羟基己基)酰胺荧光素      9889-37-2;467456-19-5        

Fmoc-Lys(5-FAM)-OH    FAM标记的Lys赖氨酸      1242933-88-5              

Fmoc-Lys(5/6-FAM)-OH  5/6-FAM标记的Lys赖氨酸    CAS: 1242933-88-5;1266666-04-9)

5(6)-TET    5/6-羧基四氯荧光素   155911-14-1                

5(6)-HEX   5/6-羧基六氯荧光素      155911-16-3                

4-Aminomethyl-6-FAM (6-CAM ) 4-氨甲基-6-羧基荧光素      326802-08-8                

4-Aminomethyl-5-FAM (5-CAM ) 4-氨甲基-5-羧基荧光素      198546-45-1                

Fura-2 ethyl ester   96331-97-4                  

BAPTA tetraethyl ester          73630-07-6                  

Calcein    207124-64-9              

Boc-Statine  58521-49-6                   

Fmoc-Statine        158257-40-0                

Diacetate-5-FAM-NHS    5-羧基二乙酰化荧光素琥珀酰亚胺酯   150206-05-6                

Diacetate-6-FAM-NHS    6-羧基二乙酰化荧光素琥珀酰亚胺酯          150206-15-8         

6-Carboxy-X-Rhodamine,SE (NHS) 6-羧基-X-罗丹明琥珀酰亚胺酯   216699-36-4       

5-Carboxy-X-Rhodamine,SE (NHS) 5-羧基-X-罗丹明琥珀酰亚胺酯   209734-74-7  

Dipiv-5-FAM-Osu (NHS)  5-羧基-二特戊酰氧基荧光素琥珀酰亚胺酯              

Dipiv-5-FAM  6-羧基荧光素二特戊酸酯                      

Dipiv-5/6-FAM-C6-OH  5/6-N-(6-羟基己基)酰胺-荧光素二特戊酸酯                       

ATTO 565 meta-isomer(1+)                              

ATTO 565 para-isomer(1+)                              

Fmoc-Glu(EDANS)-OH              193475-66-0    

 

 

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