荧光素叠氮脱硫生物素TAMRA-Azide-Desthiobiotin,荧光素-叠氮-脱硫生物素,结构式及作用
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Cy5 Biotin,荧光生物素,Biotin-Cy5,荧光生物素交联剂
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面议TAMRA-Azide-Desthiobiotin,荧光素-叠氮-脱硫生物素,结构式及作用
Desthiobiotin is a single-ring, sulfur-free analog of biotin that binds to streptavidin with nearly eual specificity but less affinity than biotin (Kd = 1011 vs. 1015M, respectively). Conseuently, desthiobiotinylated proteins can be eluted readily and specifically from streptavidin affinity resin using mild conditions based on petitive displacement with free biotin. For pull-down experiments with biological samples, this soft-release characteristic of desthiobiotin also helps to minimize co-purification of endogenous biotinylated molecules, which remain bound to streptavidin upon elution of the target protein plexes with free biotin.
Molecular weight | 1145.33 |
Chemical position | C57H82N11O14 |
CAS | N/A |
Solubility | DMSO, DMF, DCM, THF, Chloroform |
Purity | >95% |
Appearance | Red amorphous solid to red oil |
Storage conditions | -20C |
Shipping conditions | Ambient temperature |
结 构 式:
TAMRA-Azide-Desthiobiotin,荧光素-叠氮-脱硫生物素,结构式及作用
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