Desthiobiotin是一种单环的、无硫的生物素，它与链霉素结合，具有几乎相同的特异性，但与生物素(Kd = 1011和1015M)相比，其亲和力更小。因此，在与自由生物素竞争驱替的温和条件下，可以很容易地、特别地从链霉毒素亲和树脂中分离出脱硫生物素。在生物样品的下拉实验中，desthiobiotin的软释放特性也有助于减少内源性生物素化分子的共提纯，这些分子在游离生物素的目标蛋白复合物的洗脱作用下仍与链球菌肽结合。

Desthiobiotin is a single-ring, sulfur-free analog of biotin that binds to streptavidin with nearly equal specificity but less affinity than biotin (K_d = 10¹¹ vs. 10¹⁵M, respectively). Consequently, desthiobiotinylated proteins can be eluted readily and specifically from streptavidin affinity resin using mild conditions based on competitive displacement with free biotin. For pull-down experiments with biological samples, this soft-release characteristic of desthiobiotin also helps to minimize co-purification of endogenous biotinylated molecules, which remain bound to streptavidin upon elution of the target protein complexes with free biotin.

Click Chemistry Tool

DBCO Reagents

Amine Reactive

DBCO-C6-NHS Ester

• DBCO-NHS Ester
• DBCO-Sulfo-NHS Ester
• DBCO-PEG4-NHS Ester
• DBCO-PEG5-NHS Ester
• DBCO-PEG13-NHS Ester
• PC DBCO-NHS Ester
• DBCO-C6-Acid
• DBCO-Acid
• DBCO-PEG5-Acid

Carboxyl/Carbonyl Reactive

• DBCO-Amine
• DBCO-PEG4-Amine
• Sulfo DBCO-PEG4-Amine
• Sulfo DBCO-Amine

 Sulfhydryl Reactive

• Sulfo DBCO-Maleimide

DBCO-Maleimide

• Sulfo DBCO-PEG4-Maleimide
• DBCO-PEG4-Maleimide
• Bis-sulfone-PEG4-DBCO
• ThioLinker-DBCO

Fluorescent Probes

• MB 532 DBCO

MB 488 DBCO

• MB 543 DBCO
• MB 594 DBCO
• Carboxyrhodamine 110 DBCO
• TAMRA DBCO
• Cy3 DBCO
• Cy5 DBCO
• Cy5.5 DBCO
• Cy7 DBCO

 Biotinylation Reagents

• DBCO-PEG4-Biotin

WS DBCO-Biotin

• DBCO-PEG4-Desthiobiotin
• Dde Biotin-DBCO
• PC DBCO-Biotin
• WS PC DBCO-Biotin
• Diazo Biotin-DBCO

PEGylation Reagents

• DBCO-mPEG, 5 kDa

DBCO-mPEG, 10 kDa

• DBCO-mPEG, 20 kDa
• DBCO-mPEG, 30 kDa

 Bifunctional Crosslinkers

• Methyltetrazine-DBCO

Methyltetrazine-PEG12-DBCO

• DBCO-PEG4-DBCO
• TCO-PEG12-DBCO

 Miscellaneous Reagents

• DBCO-PEG4-Hydroxyl

西安凯新生物科技有限公司是国内的功能化PEG修饰纳米金及纳米金棒的供应商，我们可以提供多达十几种的活性基团及各种不同的蛋白分子或聚合物。我公司能和PEG通过双硫键偶连的Gold Nanorods的LSPR从600nm-1000nm不等，我们偶连的PEG末端带有功能活性基团可以和NH2，MAL，COOH，SH，NHS活化连接不同的蛋白或分子。

供应商：西安凯新生物科技有限公司

点击此处进入西安凯新生物科技有限公司查看

点击此处进入西安凯新生物科技有限公司查看

点击此处进入西安凯新生物科技有限公司查看