DSP（57757-57-0）功能化交联剂
时间：2018-08-07 阅读：1278
DSP（57757-57-0）功能化交联剂
西安凯新生物科技有限公司有自己的独立有机合成实验室，可以自主生产合成各种功能化蛋白交联剂，我们可以合成同功能蛋白交联剂，异功能蛋白交联剂，生物素交联剂及其他相关小分子交联剂产品，我公司自产的产品纯化纯度高达98%+以上并可以提供液相图谱来佐证纯度，并且提供相关技术指导服务。
供应商：西安凯新生物科技有限公司
研究人员经常需要将蛋白质固定在惰性表面上，用于不同的应用，包括分离和纯化ARGET大分子，选择性去除污染物，以及酶催化和化学修饰。固定化酶在金电极上广泛应用于用于检测和分析大分子的亲和生物传感器。
Chemical cross-linking is perhaps the most commonly used method for immobilizing proteins on inert surfaces. Following is a protocol for attaching a protein onto a gold surface using the chemical cross-linker dithiobis(succinimidyl propionate) . DSP is a homobifunctional, amine-reactive cross-linker. The linkage formed between DSP and the gold surface is very stable, exceeding the strength and stability of covalent silane bonds with glass. The disulfide linkage in DSP chemisorbs rapidly to gold surfaces, while the active NHS groups on either end of DSP are reactive toward primary amine groups in proteins.
化学交联也许是zui常用的固定蛋白质在惰性表面上的方法。以下是使用化学交联剂DSP二硫代氨基甲酸酯（琥珀酰亚胺丙酸酯）将蛋白质附着在金表面上的过程。DSP是一种同官能、胺反应的交联剂。在DSP与金表面之间形成的连接是非常稳定的，超过了与玻璃共价硅烷键的强度和稳定性。DSP中的二硫键化学反应迅速地到达金表面，而DSP两端的活性NHS基团对蛋白质中的伯胺基团反应。
产品名称：DSP
简介：Dithiobis succinimidyl propionate (DSP) is a homobifunctional crosslinker that is cell membrane permeable. DSP has amine-reactive N-hydroxysuccimide (NHS) esters at both ends of a cleava-ble, 8-atom (12.0 angstrom) spacer arm. DSP contains a reducible disulfide bond in the spacer arm that make it a good choice for various applications.
结构式：
Molecular Weight: 404.4
CAS #: 57757-57-0
Spacer Length: 12.0 Å
Alternative Names: 3,3′-Dithiodipropionic acid di(N-hydroxysuccinimide ester);
DSP交联剂将蛋白质附着在金表面上
所需材料
• DSP, 4 mg required per ml of DMSO coating solution
• Dimethylsulfoxide (DMSO), sufficient volume to cover and later rinse gold surface
• Protein solution: 1-2 mg/ml protein dissolved in a compatible buffer (pH 7-8, containing at least 50 mM NaCl).
Recommended buffers include PBS, MOPS and HEPES. Avoid buffers containing primary amines,such as Tris and glycine.
推荐的缓冲区包括PBS、MOP和HEPES。避免含有伯胺的缓冲液，如TrIS和甘氨酸。
Note: This procedure can be used to couple other ligands besides proteins that contain primary amines. For molecules that are not soluble in aqueous buffers, coupling can be performed in acetone, isopropyl alcohol or other solvents like DMSO, as long as amines are maintained in an uncharged state by addition of triethylamine or triethanolamine at a concentration equal to or greater than the concentration of DSP.
Procedure
1. Weigh DSP into a tube, and dissolve it in DMSO. Use 4 mg DSP per ml of DMSO necessary to cover the gold surface.
2. Incubate gold foil in dissolved DSP for 30 minutes at room temperature. 3. Rinse gold foil with DMSO and then with water. Gold foil is now activated with NHS groups. Note: Proceed directly to the next step; if protein coupling is not performed immediay, the NHS reactive groups will hydrolyze and protein coupling will be negligible.
3. Immediay add the protein solution to the activated gold foil and incubate for 1-4 hours at room temperature. Note: Incubation for longer time will not adversely affect conjugation efficiency. However, conjugation does not advance significantly after the first 1-2 hours.
4.Rinse the surface with buffer to remove cross-linker by-products (NHS leaving groups) and unconjugated protein.
Note: Free NHS groups (by-products of conjugation and hydrolysis) absorb strongly at 280 nm. Therefore, take care if attempting to determine the conjugation efficiency by measuring the change in protein concentration in solution.
应用：
•细胞裂解前的化学交联和免疫沉淀
• “固定”蛋白质相互作用，以确定弱或短暂的蛋白质相互作用
• 固定组织进行免疫染色而不损伤RNA
• 蛋白质交联通过单级反应产生生物偶联物
• 将蛋白质固定在胺涂层表面上
产品列表：
（一）：Mass Spectrometry Standards - Mass Spec Standards （质朴分析相关试剂）
Leu-Enkephalin
Gonadoliberin
Angiotensin I
Neurotensin
Peptide Calibration Standard Set
（二）：protein crosslinkers （蛋白交联相关试剂）
Protein crosslinkers （蛋白交联相关试剂）：
Heterobifunctional Crosslinkers异型双功能交联剂：
SMCC Crosslinker CAS Number: 64987-85-5
Sulfo-SMCC Crosslinker CAS Number: 92921-24-9
Sulfo-SANPAH Crosslinker CAS Number: 102568-43-4
SMPH Crosslinker
PDPH Crosslinker
MBS Crosslinker CAS Number: 58626-38-3
Sulfo-MBS Crosslinker
SPDP Crosslinker CAS Number: 68181-17-9
LC-SPDP Crosslinker
Sulfo-LC-SPDP Crosslinker
SIA Crosslinker CAS Number: 39028-27-8
SBA Crosslinker CAS Number: 42014-51-7
SMPB Crosslinker CAS Number: 79886-55-8
BMPS Crosslinker CAS Number: 55750-62-4
EMCS Crosslinker CAS Number: 55750-63-5
GMBS Crosslinker CAS Number: 80307-12-6
ANB-NOS Crosslinker CAS Number: 60117-35-3
Homobifunctional Crosslinkers同型双功能交联剂：
BS3 Crosslinker CAS Number: 82436-77-9
DSS Crosslinker CAS Number: 68528-80-3
BS2G Crosslinker
DSG Crosslinker CAS Number: 79642-50-5
DSP Crosslinker CAS Number: 57757-57-0
DTSSP Crosslinker CAS Number: 81069-02-5
Sulfo-EGS Crosslinker CAS Number: 167410-92-6
EGS Crosslinker CAS Number: 70539-42-3
DST Crosslinker CAS Number: 62069-75-4
Zero-Length Crosslinkers - EDC-HCl, Sulfo-NHS & NHS：
EDC-HCl CAS Number: 25952-53-8
Sulfo-NHS CAS Number: 106627-54-7
Deuterated Crosslinker BS3-d4
Deuterated Crosslinker BS2G-d4
Deuterated Crosslinker DSS-d4
（三）：Protein modification regents （蛋白修饰相关试剂）
Protein Modification Reagents 蛋白质修饰剂：
SATA Protein Modifier
N-Succinimidyl-S-Acetyl-Thioacetate
CAS Number: 76931-93-6
Sulfo-NHS-Acetate
CAS Number: 152305-87-8
Traut's Reagent
CAS Number: 4781-83-3
HPG
CAS Number: 24645-80-5
Iodoacetic Acid
CAS Number: 64-69-7
BMPA Modifier
CAS Number: 7423-55-4
（四）：生物素交联试剂
Biotinylation Reagents生物素化试剂
Sulfo-NHS-Biotin CAS Number: 119616-38-5
NHS-Biotin CAS Number: 35013-72-0
Sulfo-NHS-LC-Biotin CAS Number: 127062-22-0
NHS-LC-Biotin CAS Number: 72040-63-2
Sulfo-NHS-SS-Biotin CAS Number: 325143-98-4
NHS-SS-Biotin CAS Number: 142439-92-7
Biotin Hydrazide CAS Number: 66640-86-6
Biotin-HPDP CAS Number: 129179-83-5
Iodoacetyl-LC-Biotin CAS Number: 93285-75-7
（五）Immobilization Resins固定化树脂
Immobilization Resins固定化树脂
Amino-Reactive Resin
Sulfhydryl-Reactive Resin
Carbohydrate-Reactive Resin
Carboxyl-Reactive Resin
Immobilized Pepsin
Immobilized Chymotrypsin
以上资料源于西安凯新生物科技有限公司