说明书下载DATG.rar

分类名称 : 高效药物筛选,酶活性测定 产品介绍 :

产品说明  ATP酶和GTP酶催化ATP或GTP分解为ADP或GDP和磷酸盐离子。这些酶在能量运输、信号传导、蛋白质合成和细胞分化中起着关键的作用。本公司研制的ATP酶/GTP酶检测试剂盒采用微板方式，为ATP酶/GTP酶活性检测提供了一个灵敏的手段。它能在30分钟内准确检测出酶的活性。其测试方法是利用改良试剂与被释放的磷酸盐离子形成深绿色复合物的特性，用孔板吸光率阅读仪在波长600-660nm处检测。

产品特点   测量敏感、准确：能检测2 pmole的磷酸盐离子。快速、简便：单一试剂，可以在30分钟内对酶活性进行定量。高通量：在波长620nm处检测能够*地减少有色化合物的干扰；在96孔板和384孔板检测中，Z’因子大于0.7；可用于高通量自动化检测。

产品用途  1.用于ATP酶和GTP酶活性的测定。2. 用于药物开发和高通量筛选ATP酶或GTP酶抑制剂。

储藏条件  在4°C环境下，试剂和标准品能保存1年。

DESCRIPTION  ATPases and GTPases catalyze the decomposition of ATP or GTP into ADP or GDP and free phosphate ion. These enzymes play key roles in transport, signal transduction, protein biosynthesis and cell differentiation. Our ATPase/GTPase Assay Kit offers a highly sensitive method for determining ATPase/GTPase activities in a microplate format. Its proprietary formulation features a single reagent for accurate determination of enzyme activity in 30 min at room temperature. The improved reagent forms a stable dark green color with liberated phosphate, which is measured on a plate reader (600 - 660 nm).

KEY FEATURES  High sensitivity: detection of as little of 2 pmoles of free phosphate. Fast and convenient: single reagent, homogeneous “mix-and-measure” assay allows quantitation of enzyme activity within 30 minutes. Robust and amenable to HTS: detection at 620nm greatly reduces potential interference by colored compounds. Z’ factors of >0.7 are observed in 96-well and 384-well plates. Can be readily automated on HTS liquid handling systems.

APPLICATIONS  1. Determination of ATPase and GTPase activity. 2. Drug Discovery: high-throughput screen for ATPase or GTPase inhibitors.

STORAGE CONDITIONS  The reagents and standard are stable for one year when stored at 4°C.

其他 :

编号：DATG-048，DATG-200

测试：48，200T