Catalog Number :HZ290174

Quantity size : 0.1ml （dilute with pH 7.40.01 M PBS or antibody diluent ）

 

Background: Prion diseases, or transmissible spongiform encephalopathies（TSEs）, are manifested as genetic, infectious or sporadic lethal neurodegenerative disorders involving alterations of the prion protein （PrP）. Constitutively expressed in normal adult brain, cellular PrP （PrP（C）） is sensitive to proteinase K digestion and is converted to the disease form, PrPSc, through alterations in protein folding conformation, which make it resistant proteases. SPRN （shadow of prion protein）,also known as SHO or SHADOO, is a 151 amino acid cytoplasmic protein that is mainly expressed in brain. SPRN is considered a prion-like protein that has

PrP（C）-like neuroprotective activity and may act as a modulator for the biological actions of normal and abnormal PrP. In humans, mutations in the gene encoding SPRN may be associated with variant and sporadic Creutzfeldt-Jakob disease, a degenerative neurological disorder that is incurable and invariably fatal.

– Anti-Shadow/SPRN is a rabbit monoclonal antibody unconjugated

– specific for Shadow/SPRN of sheep, mouse and rat

– use for western blotting,elisa,immunoprecipitation and immunohistochemistry

– Protein G affinity chromatography purification, purity :>95%

– Isotype: IgG

– mol wt: 15kDa

 

– Western blotting 1:100-500

– Immunohistochemistry 1:100-500

– ELISA 1:500-1000

– Optimal working dilutions must be determined by the end user.

Storage: Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. The

lyophilized antibody is stable at room temperature for at least one month and for

greater than a year when kept at -20℃. When reconstituted in sterile pH 7.4 0.01M

PBS or diluent of antibody, the antibody is stable for at least six weeks at 2-4 ℃

Important Note: This product as supplied is intended for research use only, not for

use in human, therapeutic or diagnostic applications.