Protease Inhibitor Cocktail, mini-Tablet （蛋白酶抑制剂Cocktail, mini-片剂）

Description& Advantages

Endogenous proteins are produced and removed in a balanced state, so their cellular levels are generally stable under stable environmental conditions. However, protein production is greatly halted and degradation is enhanced when cells are studied in vitro. To prevent the degradation of proteins under such conditions, one can utilize a cocktail of small molecule inhibitors to block the action of proteases. Generally, protease inhibitor cocktails are used in cell lysates or tissue extracts to increase protein stability. As the name indicates, the cocktail functions to inhibit proteases that would degrade either phosphorylated or non-phosphorylated protein substrates. MedChemExpress Protease Inhibitor Cocktail (EDTA free, mini-Tablet) is a blend of 5 pan-protease inhibitors for protection of protein integrity.

Publications

• •Cell. 2020 Apr 30;181(3):621-636.e22.

• •Cell Res. 2021 Mar;31(3):345-361.

• •Adv Funct Mater. 2020, 2004886.

• •Signal Transduct Target Ther. 2021 Sep 4;6(1):333.

• •Sci Immunol. 2022 Feb 4;7(68):eabk2092.

• •Sci Adv. 2021 Mar 3;7(10):eabe8591.

• •EMBO Mol Med. 2020 Dec 7;e12798.

• •EMBO J. 2021 May 19;e106183.

• •Theranostics. 2021 Mar 4;11(10):4825-4838.

• •Theranostics. 2021 Jan 1;11(2):925-940.

• •Theranostics. 2020 Oct 30;10(26):12223-12240.

• •Theranostics. 2020 Aug 2;10(21):9830-9842.

• •Theranostics. 2020 Jul 11;10(19):8834-8850.

• •Cell Mol Immunol. 2021 Mar 11.

• •Mol Ther. 2021 Jun 7;S1525-0016(21)00312-9.

• •Acta Pharm Sin B. 2021 Jun;11(6):1592-1606.

Storage

Protease Inhibitor Cocktail (EDTA free, mini-Tablet) can be stored at 4°C for 2 years.

Protocol

1. This product can be applied in Western Blot analysis, Co-IP, pull-down, IF, IHC, kinase assay and etc.
2. One mini-Tablet can be added to 10mL extraction medium directly. Alternatively, a stock solution (10X conc. one mini-Tablet in 1mL ddH₂O) can be prepared. Vortex briefly to accelerate the dissolution. The stock solution is stable for one week when stored at 4°C, and for at least 4 weeks at -20°C.

注：产品仅用于科研