详细介绍

Leupeptin hemisulfate (Synonyms: 亮肽素)

Leupeptin hemisulfate 是一种可透过膜的巯基蛋白酶抑制剂，可抑制 Cathepsin B, Cathepsin H 和 Cathepsin L，并损害两性溶酶体的融合。Leupeptin hemisulfate 也具有抗yan作用。

生物活性

Leupeptin hemisulfate is a membrane-permeable thiol protease inhibitor that inhibits Cathepsin B, Cathepsin H and Cathepsin L, and also impairs amphisome-lysosome fusion^[1]. Leupeptin hemisulfate also exhibits anti-inflammatory effect^[2]

IC₅₀ & Target：Cathepsin^[1]

体内研究(In Vivo)

Leupeptin (0-36 mg/kg; intraperitoneal injection; for 4 hours; C57BL/6NCrl male mice) is well tolerated by the animals and produces a strong, dose-dependent increase in LC3b-II in both the total tissue extracts and the lysosome and autophagosome-enriched pellet fraction

Animal Model:	C57BL/6NCrl male mice (6-8 weeks old, 20-25 g)^[1]
Dosage:	0 mg/kg, 9 mg/kg, 18 mg/kg, 36 mg/kg
Administration:	Intraperitoneal injection; for 4 hours
Result:	Promoted the accumulation of LC3b-II in mouse liver.

分子量：475.59

Formula：C₂₀H₃₈N₆O₄.1/2H₂SO₄

CAS 号：103476-89-7

equence Shortening：Ac-LLR-CHO

中文名称：亮肽素

运输条件：Room temperature in continental US; may vary elsewhere.

储存方式：

-20°C, sealed storage, away from moisture

*In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)

溶解性数据

H₂O : 83.33 mg/mL (175.21 mM; ultrasonic and warming and heat to 60°C)

配制储备液

浓度溶剂体积质量	1 mg	5 mg	10 mg

1 mM	2.1027 mL	10.5133 mL	21.0265 mL
5 mM	0.4205 mL	2.1027 mL	4.2053 mL
10 mM	0.2103 mL	1.0513 mL	2.1027 mL

请根据产品在不同溶剂中的溶解度选择合

参考文献

[1]. Haspel J, et al. Characterization of macroautophagic flux in vivo using a leupeptin-based assay. Autophagy. 2011 Jun;7(6):629-42.

[2]. Aoyagi T, et al. Biological activities of leupeptins. J Antibiot (Tokyo). 1969 Nov;22(11):558-68.

注：产品仅用于科研

产品简介

详细介绍

Leupeptin hemisulfate (Synonyms: 亮肽素)