详细介绍

Cytochalasin D (Synonyms: 细胞松弛素D; Zygosporin A; NSC 209835)

生物活性

Cytochalasin D (Zygosporin A) is a potent actin polymerization inhibitor, could be derived from fungus. Cytochalasin D has cell-permeable activity. Cytochalasin D inhibits the G-actin–cofilin interaction by binding to G-actin. Cytochalasin D also inhibits the binding of cofilin to F-actin and decreases the rate of both actin polymerization and depolymerization in living cells. Cytochalasin D can reduce exosome release, in turn reducing the amount of survivin present in the tumour environment. Cytochalasin D induces phosphorylation and cytoplasmic retention of Yap

体外研究(In Vitro)

Cytochalasin D (3 and 10 μM; 30 min) causes retraction and arborization of COS-7 cells and conversion of F-actin from long fibers to punctate structures^[1].
Cytochalasin D (0.3, 1, 3 and 10 μM; 30 min) concentration-dependently decreases the rate of actin depolymerization in COS-7 cells^[1].
Cytochalasin D (1 μM; NIH3T3 cells) disrupts stress fibers, induces phosphorylation of Yap, while cells maintains original area and lost nuclear Yap localization

Cell Line:	COS-7 cells^[1]
Concentration:	3 and 10 μM
Incubation Time:	30 min
Result:	Caused retraction and arborization of COS-7 cells and conversion of F-actin from long fibers to punctate structures.

分子量：507.62

Formula：C₃₀H₃₇NO₆

CAS 号：22144-77-0

中文名称：细胞松弛素D

运输条件：Room temperature in continental US; may vary elsewhere.

储存方式：

Powder	-20°C	3 years
In solvent	-80°C	6 months
	-20°C	1 month

溶解性数据

DMSO : 25 mg/mL (49.25 mM; ultrasonic and warming and heat to 60°C)

配制储备液

浓度溶剂体积质量	1 mg	5 mg	10 mg

1 mM	1.9700 mL	9.8499 mL	19.6998 mL
5 mM	0.3940 mL	1.9700 mL	3.9400 mL
10 mM	0.1970 mL	0.9850 mL	1.9700 mL

请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液；一旦配成溶液，请分装保存，避免反复冻融造成的产品失效。
储备液的保存方式和期限：-80°C, 6 months; -20°C, 1 month。-80°C 储存时，请在 6 个月内使用，-20°C 储存时，请在 1 个月内使用。

参考文献

[1]. Shoji K, et, al. Cytochalasin D acts as an inhibitor of the actin-cofilin interaction. Biochem Biophys Res Commun. 2012 Jul 20;424(1):52-7.

[2]. Flanagan MD, et, al. Cytochalasins block actin filament elongation by binding to high affinity sites associated with F-actin. J Biol Chem. 1980 Feb 10;255(3):835-8.

[3]. Catalano M, et, al. Inhibiting extracellular vesicles formation and release: a review of EV inhibitors. J Extracell Vesicles. 2019 Dec 19;9(1):1703244.

[4]. Wada K, et, al. Hippo pathway regulation by cell morphology and stress fibers. Development. 2011 Sep;138(18):3907-14.

注：产品仅用于科研

产品简介

详细介绍

Cytochalasin D (Synonyms: 细胞松弛素D; Zygosporin A; NSC 209835)