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RN53-EASYspin Plus 多糖多酚/复杂植物RNA快速提取试剂盒
注:本产品仅用于科研,不可用于临床
货号# | 规格 | 价格 |
RN5301 | 50 次 | 1580.00 元 |
产品介绍:
本公司推出EASYspin无苯fen、氯仿RNA快速提取技术基础上,又研发成功基因组DNA清除柱技术可以有效清除gDNA残留,得到的RNA一般不需要DNase消化,可用于反转录PCR、荧光定量PCR等实验。裂解液迅速裂解细胞和灭活细胞RNA酶, 离心沉淀去除多糖多酚和次级代谢产物,然后裂解混合物用乙醇调节RNA结合吸附到基因组DNA清除柱,然后RNA被选择性洗脱滤过, 吸附在基因组DNA清除柱上的残留DNA无法洗脱连同柱子一起丢弃从而清除掉DNA。滤过的RNA用乙醇调节结合条件后,RNA在高离序盐状态下选择性吸附于离心柱内硅基质膜,再通过一系列快速的漂洗-离心的步骤,去蛋白液和漂洗液将细胞代谢物,蛋白等杂质去除, 最后低盐的RNase free H20将纯净RNA从硅基质膜上洗脱。
产品特点:
1.不使用有毒的苯fen,氯仿等试剂,也不需要乙醇沉淀等步骤。
2.简捷,单个样品操作一般可在25分钟内完成,快速的试剂盒。
3.成功研发基因组DNA清除柱技术可以有效清除gDNA残留,得到的RNA一般不需要DNase消化,可用于反转录PCR、荧光定量PCR等实验。
4.适应性极其广泛,可以提取包括复杂中草药如石斛/丹参/雪莲/人参、复杂淀粉种子如水稻/小麦/玉米种子、复杂果实如葡萄/蓝莓/草莓/西瓜果实、复杂抗逆植物如冬青/松针/沙棘/胡杨、复杂花卉月季/玫瑰/梅/牡丹花、复杂多糖植物紫菜/仙人掌/芦荟水稻种子等数百种国内外试剂盒提取失败的样品,使用EASYspin系列植物RNA提取试剂盒累计发表文章超过200篇。详细样品列表请参考公司主页产品介绍或者找我们索取200多篇发表文章原文。
5.多次柱漂洗确保高纯度,OD260/OD280典型的比值达1.9~2.2,无DNA残留,可用于荧光定量PCR、RT-PCR、芯片、二代测序、Northern-blot等各种实验。
使用RN53多糖多酚/复杂植物RNA快速提取试剂盒 发表部分文献
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注:本产品仅用于科研,不可用于临床