多糖多酚/复杂植物RNA快速提取试剂盒

RN5301多糖多酚/复杂植物RNA快速提取试剂盒

参考价: ¥1265

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产地
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50次
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其他
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ISO系列证书
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多糖多酚/复杂植物RNA快速提取试剂盒

参考价: ¥1265

50次 1265元 10000 瓶可售
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杭州昊鑫生物科技股份有限公司

杭州昊鑫生物科技股份有限公司

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产品简介

RN53-EASYspin Plus 多糖多酚/复杂植物RNA快速提取试剂盒
适用于快速提取植物组织细胞总RNA,使用基因组DNA清除柱技术可有效清除电泳可见gDNA残留,RNA可用于反转录PCR,荧光定量PCR等。

详细介绍

RN53-EASYspin Plus 多糖多酚/复杂植物RNA快速提取试剂盒

注:本产品仅用于科研,不可用于临床

货号#
规格
价格
RN5301
50
1580.00


产品介绍:

本公司推出EASYspin无苯fen、氯仿RNA快速提取技术基础上,又研发成功基因组DNA清除柱技术可以有效清除gDNA残留,得到的RNA一般不需要DNase消化,可用于反转录PCR、荧光定量PCR等实验。裂解液迅速裂解细胞和灭活细胞RNA酶, 离心沉淀去除多糖多酚和次级代谢产物,然后裂解混合物用乙醇调节RNA结合吸附到基因组DNA清除柱,然后RNA被选择性洗脱滤过, 吸附在基因组DNA清除柱上的残留DNA无法洗脱连同柱子一起丢弃从而清除掉DNA。滤过的RNA用乙醇调节结合条件后,RNA在高离序盐状态下选择性吸附于离心柱内硅基质膜,再通过一系列快速的漂洗-离心的步骤,去蛋白液和漂洗液将细胞代谢物,蛋白等杂质去除, 最后低盐的RNase free H20将纯净RNA从硅基质膜上洗脱。

产品特点:

1.不使用有毒的苯fen,氯仿等试剂,也不需要乙醇沉淀等步骤。
2.简捷,单个样品操作一般可在25分钟内完成,快速的试剂盒。
3.成功研发基因组DNA清除柱技术可以有效清除gDNA残留,得到的RNA一般不需要DNase消化,可用于反转录PCR、荧光定量PCR等实验。
4.适应性极其广泛,可以提取包括复杂中草药如石斛/丹参/雪莲/人参、复杂淀粉种子如水稻/小麦/玉米种子、复杂果实如葡萄/蓝莓/草莓/西瓜果实、复杂抗逆植物如冬青/松针/沙棘/胡杨、复杂花卉月季/玫瑰/梅/牡丹花、复杂多糖植物紫菜/仙人掌/芦荟水稻种子等数百种国内外试剂盒提取失败的样品,使用EASYspin系列植物RNA提取试剂盒累计发表文章超过200篇。详细样品列表请参考公司主页产品介绍或者找我们索取200多篇发表文章原文。
5.多次柱漂洗确保高纯度,OD260/OD280典型的比值达1.9~2.2,无DNA残留,可用于荧光定量PCR、RT-PCR、芯片、二代测序、Northern-blot等各种实验。


使用RN53多糖多酚/复杂植物RNA快速提取试剂盒 发表部分文献

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4.     芜菁:Isolation and Functional Characterisation of the Genes Encoding Δ8-Sphingolipid       Desaturase from Brassica rapa. Journal of Genetics and Genomics Volume 39, Issue 1, January    2012,    Pages 47–59


5.     芜  菁 1 :EXPRESSION, DIVERGENCE AND EVOLUTION OF THE CALEOSIN GENE FAMILY IN BRASSICA RAPA. Arch. Biol. Sci., Belgrade, 65 (3), 863-876, 2013 DOI:10.2298/ABS1303863H


6.     番茄叶:Effect of Low Temperature Stress on the Expression of ProDH Gene and the Activities of the Proline Dehydrogenase in Leaves of Tomato Seedling. Chinese Agricultural Science  Bulletin 2012,28(10):132-135


7.     栀子叶:Isolation of High Quality Total RNA from Gardenia jasminoides Eills.Chinese Agricultural    Science Bulletin.2012, 28(27):194-198


8.     油桐果实:Cui Qinqin, Han Xiaojiao, Chen Yicun, Zhan Zhiyong, Lin Liyuan, Wang Yangdong.     Isolation and Expression Characteristics of Biotin Carboxyl Carrier Protein Coding Gene(VfBCCP)  from Vernicia fordii.SCIENTIA SILVAE SINICAE. 2012, 48(8): Available online August


9.     油桐果实1:Selection of Reliable Reference Genes for Gene Expression Studies Using Real-Time    PCR in Tung Tree during Seed Development. PLoS ONE, 2012, 7(8): e43084


10. 紫菜:Molecular cloning and expression analysis of ribosomal protein S7 gene from Porphyra    haitanensis. JOURNAL OF FISHERIES OF CHINA, 2011, 35(12):1814-1821


11. 石斛:Molecular characterization of a mitogen-activated protein kinase gene DoMPK1 in Dendrobium officinale. Acta Pharmaceutica Sinica, 2012, 47 (12): 1703-1709


12. 石斛1:ESTs Analysis Reveals Putative Genes Involved in Symbiotic Seed Germination in Dendrobium officinale. Symbiotic Germination Genes in D. officinale. August 2013 | Volume 8 | Issue 8 | e72705


13. 大豆:RNA-seq Analysis Reveals Ethylene-Mediated Reproductive Organ Development and Abscission in Soybean(Glycine max L. Merr.). Plant Mol Biol Rep, 2012, published online: 4 Dec, 2012


14. 大豆1:Construction of ethylene regulatory network based on the phytohormones related gene transcriptome profiling and prediction of transcription factor activities in soybean. Acta Physiol Plant, 2012, published online: 12 Dec, 2012


15. 红花玉兰:Expression Analysis of MAwuAG in Different Organs and Developmental Stages of Magnolia wufengensis. Chinese Bulletin of Botany, 2013, 48 (2): 1–5


16. 毛桃:Cloning and Phylogeny Analysis of PpAP2 Floral Homologous Genes in Peach. Chinese Agricultural Science Bulletin, 2013, 29(7): 99-104


17. 五倍子:Cloning and characterisation of a phenylalanine ammonia-lyase gene from Rhus chinensis. Plant Cell Rep, 2013, published online:15 March, 2013


18. :五倍子1:Cloning, characterization and expression of chalcone synthase from medicinal plant Rhus chinensis.J. Plant Biochem. Biotechnol. DOI 10.1007/s13562-013-0231-9


19. 青杄 :cDNA Cloning and Bioinformatic Analysis of the sPPa1 Gene form Picea wilsonii. Plant Science Journal, 2012, 30(40): 394-401


20. 青杄 1:cDNA Cloning and Bioinformatic Analysis of PsbO Gene from Picea wilsonii.Life Science Research, 2012, 16(3): 201-206


21. 青杄 2:Cloning and Tissue Expression Analysis of PwPSAF in Picea wilsonii. SCIENTIA SILVAE SINICAE. Vol. 49,No. 10, Oct. 2013.


22. 洋葱:Molecular Cloning and Transcriptional Analysis of the Putative AGAMOUS Homolog AcAG in Onion (Allium cepa. Plant Mol Biol Rep, DOI 10.1007/s11105-013-0607-y


23. 木瓜:XsFAD2 gene encodes the enzyme responsible for the high linoleic acid content in oil accumulated in Xanthoceras sorbifolia seeds. JOURNAL ARTICLE. 2013-6-17.


24. 木瓜1:Two novel diacylglycerol acyltransferase genes from Xanthoceras 2 sorbifolia are responsible for its seed oil content. GENE-38688; No. of pages: 9; 4C:


25. 柑橘:Efficient auto-excision of a selectable marker gene from transgenic citrus by combining the Cre/loxP system and ipt selection. Plant Cell Rep, DOI 10.1007/s00299-013-1470-x


26. 柑橘1:Expression Analysis of Three Phloem-specific Promoters in Transgenic Poncirus trifoliata. Acta Horticulturae Sinica. 2014, 41(1): 1–8.


27. 柑橘2: Activation of three pathogen-inducible promoters in transgenic citrus (Citrus sinensis Osbeck) after Xanthomonas axonopodis pv. citri infection and wounding. Plant Cell Tiss Organ Cult. DOI 10.1007/s11240-013-0423-y.


28. 茶梅花瓣:Comparison and Analysis of Methods of Extracting Total RNA from Petals of Camellia sasanqua. Chinese Agricultural Science Bulletin.2013,29(28):129-133.


29. 栀子:Isolation of High Quality Total RNA fromGardenia jasminoides Eills. Chinese Agricultural Science Bulletin. 2012, 28(27):194-198


30. 丹参:Genome-wide analysis and molecular dissection of the SPL gene family in Salvia miltiorrhiza. 2014 Jan;56(1):38-50. doi: 10.1111/jipb.12111. Epub 2013 Nov 20.


31. 牡丹:Transcriptome Comparison Reveals Key Candidate Genes Responsible for the Unusual Reblooming Trait in Tree Peonies. Genes Responsible for Reblooming in Tree Peonies. November 2013 | Volume 8 | Issue 11 | e79996


32. 东南景天:Role of sulfur assimilation pathway in cadmium hyperaccumulation by Sedum alfredii Hance. Ecotoxicology and Environmental Safety. Volume 100, February 2014, Pages 159–165.


33. 山苍子:Identification of appropriate reference genes for normalizing transcript expression by quantitative real‑time PCR in Litsea cubeba. TECHNICAL NOTE. Mol Genet Genomics (2013) 288:727–737, DOI 10.1007/s00438-013-0785-1


34. 木本植物:Heterologous gene silencing induced by tobacco rattle virus (TRV) is efficient for pursuing functional genomics studies in woody plants. ORIGINAL PAPER. Plant Cell Tiss Organ Cult, DOI 10.1007/s11240-013-0393-0


35. 棉花:Analysis of sea-island cotton and upland cotton in response to Verticillium dahliae infection by RNA sequencing. Sun et al. BMC Genomics 2013, 14:852 /1471-2164/14/852.






注:本产品仅用于科研,不可用于临床

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