下载制药通APP了解更多制药 行业资讯

打开APP
商品详情
11

Forskolin试剂

参考价:600.00 - ¥1500.00

具体成交价以合同协议为准

杭州市

Forskolin试剂

参考价: ¥600.00~¥1500.00

10 mM * 1 mL in DMSO 660.00元 10000 可售
5 mg 600.00元 10000 可售
10 mg 810.00元 10000 可售
50 mg 1500.00元 10000 可售
x

杭州昊鑫生物科技股份有限公司

产品型号:HY-15371     厂商性质:代理商
天城路176号白云大厦2幢1003-1005室
进入商铺全部商品

产品简介

Forskolin试剂 是一种有效的腺苷酸环化酶 (adenylate cyclase) 激活剂,对于 I 型腺苷酸环化酶,IC50 为 41 nM,EC50 为 0.5 μM。Forskolin 也是一种细胞内 cAMP 形成的诱导剂。Forskolin 诱导多种细胞类型的分化并激活孕烷 X 受体 (PXR) 和 FXR。

产品详情

注:本产品仅用于科研,不可用于临床

Forskolin试剂简介

Forskolin (Coleonol) 是一种有效的腺苷酸环化酶 (adenylate cyclase) 激活剂,对于 I 型腺苷酸环化酶,IC50 为 41 nM,EC50 为 0.5 μM。Forskolin 也是一种细胞内 cAMP 形成的诱导剂。Forskolin 诱导多种细胞类型的分化并激活孕烷 X 受体 (PXR) 和 FXR。Forskolin 对心脏产生正性肌力作用,并具有血小板抗凝集和降压作用。Forskolin 也可诱导细胞自噬 (autophagy)。





Forskolin试剂生物活性

Forskolin (Coleonol) is a potent adenylate cyclase activator with an IC50 of 41 nM and an EC50 of 0.5 μM for type I adenylyl cyclase[1]. Forskolin is also an inducer of intracellular cAMP formation[2]. Forskolin induces differentiation of various cell types and activates pregnane X receptor (PXR) and FXR[3]. Forskolin exerts a inotropic effect on the heart, and has platelet antiaggregatory and antihypertensive actions. Forskolin also induces autophagy[4][5].

IC50 & Target

IC50: 41 nM (Adenylyl cyclase)[1]
EC50: 0.5 μM (Adenylyl cyclase)[1]

体外研究
(In Vitro)

Forskolin (Coleonol) is also a potent exosome biogenesis and/or secretion activator in prostate cancer (PC) cells[8].
Forskolin (Fsk) is a naturally occurring diterpene isolated from Coleus forskholii, directly activates adenylyl cyclase (AC) through its catalytic subunit to increase intracellular levels of cyclic adenosine monophosphate (cAMP)[1].
Forskolin (Fsk) affects the proliferation of the human T-cell lines such as Kit 225 and MT-2. Forskolin treatment inhibits the proliferation of both Kit 225 and MT-2 cells in a dose-dependent manner with an IC50 equal to ~5 μM Fsk. Forskolin treatment (10-100 μM) increases cAMPi levels ~5- to 20-fold above basal levels, which reache maximum levels between 50-100 μM Forskolin[6].

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

体内研究
(In Vivo)

The Forskolin (Coleonol)-treated Mrp4-/- mice shows an increased number of Ki67-positive and cleaved caspase 3-positive ECs, a significant decrease in the amount of pericyte coverage, and a reduced number of empty sleeves. In pups exposed to hyperoxia (75% oxygen) from P7 to P12, the Mrp4-/- mice shows a significant increase in the unvascularized retinal area[2].
The average blood glucose in the healthy rat group is 102.12±1.94 mg/dL, 101.25±3.56 for control group and 103±2.08 in forskolin group. The data shows that glucose levels at the end of the study are lower in forskolin group, with a significant difference according to the statistical tests applied (p=0.03)[7].

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

分子量

410.50

性状

Solid

Formula

C22H34O7

CAS 号

66575-29-9

中文名称

毛喉素

结构分类
  • Terpenoids

  • Diterpenoids

来源
  • Plants

  • Compositae

  • Gochnatia polymorpha (Less) Cabr.

  • Plants

  • Labiatae

运输条件

Room temperature in continental US; may vary elsewhere.

储存方式

4°C, protect from light

*In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)

溶解性数据
In Vitro:

DMSO : 100 mg/mL (243.61 mM; Need ultrasonic)

配制储备液
浓度溶剂体积质量1 mg5 mg10 mg
1 mM2.4361 mL12.1803 mL24.3605 mL
5 mM0.4872 mL2.4361 mL4.8721 mL
10 mM0.2436 mL1.2180 mL2.4361 mL
*

请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液;一旦配成溶液,请分装保存,避免反复冻融造成的产品失效
储备液的保存方式和期限:-80°C, 6 months; -20°C, 1 month (protect from light)。-80°C 储存时,请在 6 个月内使用,-20°C 储存时,请在 1 个月内使用。

In Vivo:

请根据您的实验动物和给药方式选择适当的溶解方案。以下溶解方案都请先按照 In Vitro 方式配制澄清的储备液,再依次添加助溶剂:

——为保证实验结果的可靠性,澄清的储备液可以根据储存条件,适当保存;体内实验的工作液,建议您现用现配,当天使用; 以下溶剂前显示的百
分比是指该溶剂在您配制终溶液中的体积占比;如在配制过程中出现沉淀、析出现象,可以通过加热和/或超声的方式助溶

  • 1.


    请依序添加每种溶剂: 10% DMSO    40% PEG300    5% Tween-80    45% saline

    Solubility: ≥ 2.5 mg/mL (6.09 mM); Clear solution


  • 2.


    请依序添加每种溶剂: 10% DMSO    90% corn oil

    Solubility: ≥ 2.5 mg/mL (6.09 mM); Clear solution






在线咨询 热线电话
免费会员