Cy5 Picolyl Azide,Cy5-N3,Alexa Fluor 647
目前Cyanine系列染料以两种异构体的形式存在，一种是非磺化花青素（non-sulfonated cyanines），一种是磺化花青素（sulfonatedcyanine）。两者的光谱性质几乎*，因此大部分应用可相互替换。
Cy5 Picolyl Azide,Cy5-N3,Alexa Fluor 647
产品介绍
Cy5 Picolyl Azide is an advanced fluorescent probe that incorporates a copper-chelating motif to raise the effective concen*ration of Cu(I) at the reaction site. The chelating effect of picolyl azide moiety to raise the effective concen*ration of copper at the reaction site is so great that it leads to an increase of signal intensity of up to 40-fold.
In addition, the use of Cy5 Picolyl Azide instead of conventional Cy5-Azide allows for at least a tenfold reduction in the concen*ration of the copper catalyst without sacrificing the efficiency of labeling.
In summary, the in*roduction of a picolyl moiety into an azide probe leads to a substantial increase in the sensitivity of alkyne detection. This will be of special value for the detection of low abundance targets or where significant increase in signal intensity is desired.
Abs/Em Maxima | 647/663 nm |
Extinction coefficient | 250,000 |
Flow cytometry laser line | 633 or 635 nm |
Microscopy laser line | 633 or 635 nm |
Spectrally similar dyes | Alexa Fluor® 647, Atto™ 647, CF™ 647 Dye, DyLight™649 |
Molecular weight | 953.15 |
CAS | N/A |
Solubility | Water, DMSO, DMF, MeOH |
Purity | >95% (HPLC) |
Appearance | Blue solid |
Storage conditions | -20C |
Shipping conditions | Ambient temperature |
结构式：
Cy5 Picolyl Azide,Cy5-N3,Alexa Fluor 647
西安凯新生物科技有限公司
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